poly Lactose File Name C:\Documents and Settings\Ayres Research Group\My Documents\Leeanne\poly Lactose.afe6 Collection Version 6.0.1.10 Collection Operator AYRESGPC\Ayres Research Group (AYRESGPC\Ayres Research Group (Ayres Research Group)) Processing Operator AYRESGPC\Ayres Research Group (Ayres Research Group) sample dn/dc 1.850000000e-001 A2 0.000000000e+000 UV Ext. Coef. 0.000000000e+000 Mark-Houwink-Sakurada K 0.000000000e+000 Mark-Houwink-Sakurada a 0 Reference Wavelength 0.000000000e+000 Reference Temperature 2.500000000e+001 Concentration 7.700000000e+000 Collection procedure template Despiking Procedure template Define Baselines template Alignment template Broaden template Define Peaks template Molar Mass & Radius from LS template Results fitting : Molar Mass Results fitting : rms radius Results fitting : mean square radius Results fitting : Hydrodynamic Radius Results fitting : Intrinsic Viscosity Mark-Houwink-Sakurada template Distribution Analysis template molar mass vs volume testing testing Abscissa Units min Concentration Source RI Flow Rate 1.000000000e+000 thf System solvent Reference Refractive Index 1.402938872e+000 Temperature Correction Enabled no Reference Temperature 2.500000000e+001 Reference Wavelength 6.328000000e+002 Refractive Index 1.402273917e+000 Rayleigh Ratio 3.690183168e-006 Viscosity 4.550199963e-001 Refractive Index Model Model Name Polynomial Equation N(λ) = 1.40282 + 0.0038323 λ² - 0 λ^4 - 0- 0.000378058 * 25 Rayleigh Ratio Model Model Name Corrected Lambda^4 Equation R(λ, T) = 4.28e-006 * (632.8/λ)^4 * (N(λ)/N(632.8))²[(N(λ) - 1)/(N(632.8) - 1)]² Viscosity Model Model Name Linear Equation η(T) = 0.00456 + -4.9e-005 * (T - 25) Thermal Expansion Model Model Name Fixed Equation fixed at 0 @ 0 °C injector Injected Volume 0.000000000e+000 Auto Inject Signal no Flow Marker 0.000000000e+000 Mark-Houwink-Sakurada a 0.000000000e+000 Mark-Houwink-Sakurada K 0.000000000e+000 Conventional Calibration Function Fit Standard Error 0 Conventional Calibration Function Fit Adjusted RҢ 0 Universal Calibration Function Fit Standard Error 0 Universal Calibration Function Fit Adjusted RҢ 0 Calibration Technique none Conventional Calibration Function Universal Calibration Function Plate Count 0 Asymmetry Factor 0 Resolution 0 miniDAWN TREOS Temperature Control no Band Broadening Correction n/a Disable Data Collection no Physical Instrument Name miniDAWN TREOS Physical Instrument Location WYATT-333-TS Batch Mode no Cell Type Fused Silica Wavelength 6.560000000e+002 Calibration Constant 6.105000000e-005 Comet Cell Cleaner no Divide raw LS signal by laser monitor yes Divide raw LS signal by forward laser monitor no QELS Fiber Replaces Detector 0 Polarization Option no Temperature Controlled Line no Heated Line Temperature 0.000000000e+000 QELS Enabled no 1 46.6 0.745 2 90.0 1.000 3 133.4 0.837 Optilab rEX Temperature Control yes Temperature 2.520000076e+001 Band Broadening Correction n/a Disable Data Collection no Physical Instrument Name Optilab rEX Physical Instrument Location WYATT-869-rEX Batch Mode no Wavelength 6.580000000e+002 Calibration Constant 3.460689913e-005 ViscoStar Temperature Control yes Temperature 2.500000000e+001 Band Broadening Correction n/a Disable Data Collection no Physical Instrument Name ViscoStar Physical Instrument Location Wyatt-222-V2 Batch Mode no Dilution Factor 0.4976 Collect Using Specific Viscosity no Capillary Volume 0.000000000e+000 Generic Pump Injector 0.00000000 Injector Generic Column 0.00000000 Generic Column miniDAWN TREOS 0.00000000 miniDAWN TREOS ViscoStar 0.00000000 ViscoStar Optilab rEX 0.00000000 Not Specified miniDAWN TREOS Collection Time Monday January 27, 2014 03:10:18 PM Eastern Daylight Time Processing time Monday January 27, 2014 04:03:03.421 PM Eastern Daylight Time Despiking Level None Basic Collection Collection Script File Name Inject to Collect Delay 0.000000000e+000 Collection Duration 3.000000000e+001 LS Instrument Collection Interval 1.000000000e+000 QELS Instrument Collection Interval 3.000000000e+000 Wait for Auto-inject Signal yes Define Baselines detector 1 5.35161311 0.05064296 29.54693434 0.05071696 manual x, auto y detector 2 3.99486612 0.02956663 29.58462175 0.02963771 manual x, auto y detector 3 3.46724229 0.03919499 29.81074625 0.03912627 manual x, auto y differential refractive index data 4.65650660 -0.00000011 29.67079827 -0.00000006 manual x, auto y Differential Pressure 7.02336773 -0.00028685 29.89007280 0.00007847 manual x, auto y Band Broadening Reference Instrument Optilab rEX Broaden Band Broadening Algorithm ASTRA 6 Define Peaks Peak Name Peak 1 Peak 2 Start 1.863637382e+001 2.310172280e+001 End 2.155176695e+001 2.435644895e+001 Light Scattering Model Zimm Zimm Fit Degree 1 1 dn/dc 2.000000000e-002 2.000000000e-002 dn/dc Ref. Temp. 2.500000000e+001 2.500000000e+001 Modifier dn/dc 0.000000000e+000 0.000000000e+000 Modifier dn/dc Ref. Temp. 2.500000000e+001 2.500000000e+001 A2 0.000000000e+000 0.000000000e+000 UV Ext. Coef. 0.000000000e+000 0.000000000e+000 UV Ext. Coef. Ref. Temp. 2.500000000e+001 2.500000000e+001 Modifier UV Ext. Coef. 0.000000000e+000 0.000000000e+000 Modifier UV Ext. Coef. Ref. Temp. 2.500000000e+001 2.500000000e+001 Real Refractive Index 0 0 Imaginary Refractive Index 0 0 Shell Thickness 0.000000000e+000 0.000000000e+000 Shell Real Refractive Index 0 0 Shell Imaginary Refractive Index 0 0 Rod radius 0.000000000e+000 0.000000000e+000 Molecular Standard n/a n/a Concentration 0.000000000e+000 0.000000000e+000 Concentration Ref. Temp. 2.500000000e+001 2.500000000e+001 Injected Mass 0.000000000e+000 0.000000000e+000 Molar Mass 0.000000000e+000 0.000000000e+000 Intrinsic Viscosity 0.000000000e+000 0.000000000e+000 Intrinsic Viscosity Ref. Temp. 2.500000000e+001 2.500000000e+001 Mark-Houwink-Sakurada K 0.000000000e+000 0.000000000e+000 Mark-Houwink-Sakurada a 0 0 Flory-Fox Equation Phi Parameter 0 0 Ptitsyn-Eizner Equation Epsilon Parameter 0 0 Viscometry Model Huggins Huggins Huggins Equation Parameter 0 0 Kraemers Equation Parameter 0 0 Radius 3.000000000e+000 3.000000000e+000 Radius Type rms rms Normalization Normalization Peak 1 Percent to Keep 2.500000000e+001 Molar Mass & Radius from LS Enabled Peaks 1 2 Enabled Detectors 1 2 3 Results Fitting Procedure laser monitor average 9.997348672e-001 Forward Monitor Average 7.782304427e-001 laser current average 9.497973228e-002 laser voltage average 1.299427401e-003 Calculated Mass 5.558075212e+002 1.391879378e+003 Mass Recovery n/a n/a Mass Fraction 2.853680031e+001 7.146319969e+001 Mark-Houwink-Sakurada a -3.814517329e-001 Mark-Houwink-Sakurada K 1.048220231e+001 Mn 7.029554576e+004 1.470731138e+003 Mp 7.508180086e+004 1.526272789e+003 Mv 7.763336238e+004 1.695121151e+003 Mw 1.315414814e+005 6.310657377e+003 Mz 4.504943573e+005 8.003743862e+004 Mz+1 9.392543927e+005 1.246781056e+005 Mw/Mn 1.871263392e+000 4.290830061e+000 Mz/Mn 6.408576139e+000 5.442017005e+001 M(avg) 3.442345702e+005 1.670184234e+003 Rn n/a n/a Std Dev Rn n/a n/a Rw n/a 5.255664934e+001 Std Dev Rw n/a 9.094424022e+001 Rz n/a 1.369361600e+002 Rh(n) 2.542319858e+000 4.925275212e-001 Std Dev Rh(n) 6.783989200e-001 1.304460277e-001 Rh(w) 3.111856971e+000 6.818219058e-001 Std Dev Rh(w) 1.622486178e+000 5.998336449e-001 Rh(z) 5.478736809e+000 2.642852797e+000 ηn 1.566321460e+000 5.820283692e-001 ηw 1.861617472e+000 6.955265329e-001 ηz 3.051581326e+000 1.846095384e+000 Rh(avg) 2.915910054e+000 4.818618933e-001 η(avg) 1.785262410e+000 7.937208979e-001 Average concentration 9.519181981e-002 5.552231199e-001 R(avg) 6.047318473e+001 RMS conformation plot slope 4.559221241e-002 RMS Conformation Plot y-intercept 1.871617895e+000 Rh conformation plot slope 4.098947345e-001 Rh Conformation Plot y-intercept -1.547828391e+000 RMS radius vs. Rh plot slope 1.978104270e+003 RMS Radius vs. Rh Plot y-intercept 8.501709199e+004